Tissue Culture and Transformation

 

  • Tissue culture and transformation
    1. Chinese cabbage tissue culture: the factors can influence Chinese cabbage in vitro
      cotyledons and hypocotyls explants regeneration. They include best hormone
      combination and concentration, best gelling agent and other factors. Research on best
      conditions for rootdevelopment from regenerated shoots. Research on acclimation
      methods for regenerated plantlets.

    2. Transformation: after infection, research the factors influence regeneration frequency.
      On regenerated plantlets, focus on how to improve transformation frequency.
  • Progress
    1. Established a high plant regeneration system from inbred line Chinese cabbage
      cotyledons explants.

    2. Got a relatively high regeneration frequency from agrobacterium infected explants.
      Undergoing on transformation frequency improvement and certification.



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